Sample collection
Pigment samples were collected on the R/V Roger A. Revelle, Survey II, cruise. The sample collection occurred during the same period as a SeaSoar survey. Water was collected from the ship’s surface seawater intake system. Duplicate 2L aliquots were filtered onto GF/F Whatman filters. Filters were frozen and stored in a -80°C freezer until analyzed on shore.

Sample analysis
Samples were extracted in 3mL of 100% acetone with the addition of 0.1 mL of internal standard canthaxanthin. Samples were sonicated for 90 seconds and extracted for 12 hours in a -20°C freezer. They were then pre-concentrated under constant flow of nitrogen for 10 minutes. Phytoplankton pigment concentrations were determined using a High Pressure Liquid Chromatography (HPLC) system composed of a Perkin-Elmer pump, an Ultrasphere analytical column (150 mm x 4.6 mm), and a SpectraSystem UV2000 absorption detector (dual wavelength, at 436 & 665 nm). The mobile solvent system method was adapted from Wright and Jeffrey (Wright, S. W., and S. W. Jeffrey, "High-resolution HPLC system for chlorophylls and carotenoids of marine phytoplankton", in Phytoplankton Pigments in Oceanography: Guidelines to Modern Methods, eds. S. W. Jeffrey, R. F. Mantoura, and S. W. Wright, Unesco Pub., Paris, pp. 327-341, 1997). The instrument was calibrated using commercial pigment standards (Sigma Chemicals and VKI, Denmark).

The pigments studied include:

• chlorophyll-a
• fucoxanthin
• 19'-hexanoyloxyfucoxanthin
• chlorophyll-c₁ and -c₂
• chlorophyll-c₃
• 19'-butanoyloxyfucoxanthin
• chlorophyll-b
• diadinoxanthin

Webpage by Jasmine S. Bartlett, Oregon State University.